An animal study on the effectiveness of platelet-rich plasma as a direct pulp capping agent.

Direct pulp capping (DPC) is a conservative approach for preserving tooth vitality without requiring more invasive procedures by enhancing pulp healing and mineralized tissue barrier formation. We investigated the effectiveness of Platelet Rich Plasma (PRP) vs. Mineral Trioxide Aggregate (MTA) as a DPC agent. Forty-two teeth from three mongrel dogs were divided into two equal groups. After three months, the animals were sacrificed to evaluate teeth radiographically using cone-beam computerized tomography, histopathologically, and real-time PCR for dentin sialophosphoprotein (DSPP), matrix extracellular phosphoglycoprotein (MEPE), and nestin (NES) mRNA expression. Radiographically, hard tissue formation was evident in both groups without significant differences (p = 0.440). Histopathologic findings confirmed the dentin bridge formation in both groups; however, such mineralized tissues were homogenous without cellular inclusions in the PRP group, while was osteodentin type in the MTA group. There was no significant difference in dentin bridge thickness between the PRP-capped and MTA-capped teeth (p = 0.732). The PRP group had significantly higher DSPP, MEPE, and NES mRNA gene expression than the MTA group (p < 0.05). In conclusion, PRP enables mineralized tissue formation following DPC similar to MTA, and could generate better cellular dentinogenic responses and restore dentin with homogenous architecture than MTA, making PRP a promising alternative DPC agent.

Knowledge and attitudes toward evidence-based cariology and restorative dentistry among Egyptian dental practitioners: a cross-sectional survey.

BACKGROUND: This is the first study to assess Egyptian dental practitioners' knowledge about conservative caries management approaches and investigate whether this knowledge transfers into clinical practice and the barriers to translating research into evidence-based practice. METHODS: A sample of dental practitioners was surveyed using an online questionnaire. Convenience and snowball sampling were used to collect data from February to June 2022. We included graduated dentists from Egyptian universities who practiced in Egypt. Data were analyzed with descriptive statistics, and the associations between variables were checked using Kruskal Wallis and Chi-Square tests. RESULTS: This study included 396 participants from throughout Egypt. There were significant correlations between specialty and participants' knowledge and behaviors toward evidence-based caries management (p = 0.002) and between specialization and tools used to detect carious lesions (p < 0.001). Most participants (59.1%) used G.V Black's classification, and (80.8%) removed caries based on the feature of dentin hardness and color, whereas (67%) removed caries until hard dentine remained. The participants' primary hurdle to staying up-to-date was their belief that the newly gained information would not be clinically applicable due to a lack of equipment or working in low-economic areas. Patient-related barriers were the major obstacles for participants in implementing evidence-based practice. CONCLUSION: Egyptian dentists did not fully embrace minimal invasive approaches for caries management, and practitioners' experiences continue to shape decision-making. It emphasizes the imperative to practically educate dentists using effective knowledge translation dissemination to promote evidence adoption in daily practice and advocate value-based dental care to address the economic crisis's impact on Egypt's healthcare.

Chlorhexidine to improve the survival of ART restorations: A systematic review and meta-analysis.

OBJECTIVES: Our aim was to systematically assess the efficacy of chlorhexidine (CHX) as a cavity pre-treatment or restoration mix-in on the survival of ART restorations. DATA: We included randomized controlled trials that assessed the effect of cavity pretreatment with CHX or the restoration mix-in of CHX on the survival of ART restorations (test group) versus a similar restorative approach without CHX. Risk of bias was assessed using Cochrane's randomized trial quality assessment Tool (RoB 2.0). Random-effects meta-analysis was conducted, with mean Odds Ratios and 95 % confidence intervals (OR, 95 % CI) as effect estimates. The certainty of the evidence was assessed according to the Grading of Recommendations Assessment, Development, and Evaluation (GRADE) system. SOURCES: Medline, Web of Science, Cochrane Central were searched. Eligible studies were in- or excluded and data extracted for included studies by two reviewers independently. STUDY SELECTION: Four studies with 261 patients (mean age 3.8-14.6 years) and 467 ART restorations were included. All studies showed some concerns about the risk of bias. Three studies involving a total of 167 restorations in the CHX group and 188 restorations in the control group, followed up for one year, were submitted to meta-analysis. There were no significant differences between CHX vs. control (OR = 0.79, 95 % CI [0.26, 2.40], P = 0.68, I2 = 3%, P = 0.35). The strength of the evidence was estimated as low. CONCLUSIONS: Based on very limited data, CHX pre-treatment or restoration mix-in did not have any significant benefit for survival of ART restorations. CLINICAL SIGNIFICANCE: There is a lack of evidence regarding the influence of chlorhexidine on the survival of ART restorations. Our results revealed that there was no significant difference in the survival of ART restorations when CHX was used as a cavity pre-treatment or mix-in.